Tobacco transcriptomic changes induced by C. sph. MVOC

Transciptomic changes in tobacco (Nicotiana tabacum cv. Samsun) following exposure to microbial volatile organic compounds (MVOCs) emitted by Cladosporium sphaerospermum isolate TC09 (NRRL 67603) were investigated. Germinated seedling plants were cultured in Magenta GA7 vessels containing Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose with and without fungal cultures that were included in the vessel but placed in a separated container. Ten days after the onset of MVOC exposure, tissue samples including the apical meristem and terminal leaves were collected and subject to RNA sequencing and transcriptome analysis using available tomato genome as reference. Our study revealed massive number of differentially expressed genes (DEGs) associated with diverse biological processes including cell expansion and cell cycle, photosynthesis, phytohormone homeostasis and defense responses as a consequence of short-term exposure to potent MVOCs. Overall design: Two exposure treatments (control without exposure and MVOC exposed), 4 biological replicates per treatment, 3 seedling plants er replicate