PLA was performed in HEK 293 cells transfected with ASRGL1 and TDP-43 plasmids and incubated with antibodies: no primary, only TDP-43, only ASRGL1, TDP-43 + IgG and ASRGL1 + TDP-43.
Proximal proteins to S1PR1-WT and S1PR1-TM4 (a swap mutant of the transmembrane domain 4 with that of S1PR3) were obtained in human umbilical vein endothelial cells by a TurboID-catalyzed proximity bi
