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Deep sequencing data for sequence specificity on four ScCas9-based variants in rice protoplast

NIAID Data Ecosystem2026-05-02 收录

CRISPR-Cas9脱靶

植物基因编辑

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP528161 数据链接链接失效反馈

官方服务：

资源简介：

compare the sequence specificity of Sc++, ScRN++, SpcRN+, and SpcRN++ by mismatch tolerance assay in rice protoplast

应用场景：

创建时间：

2024-08-24

CRISPR-Cas9脱靶

DNA断裂检测

We identified robust cleavage by non-canonically-targeted SpCas9 that target protospacer with a few bases from the canonical NGG PAM in biochemistry assay. To determine whether this spaced SpCas9 clea

NIAID Data Ecosystem80

Multiallelic, targeted mutagenesis of magnesium chelatase with CRISPR-Cas9 provides a rapidly scorable phenotype in highly polyploid sugarcane

植物基因编辑

遗传改良

Study aimed to achieve high levels of multiallelic targeted mutagenesis in sugarcane using CRISPR/Cas9 to target the chlorophyll synthesis gene magnesium chelatase.

NIAID Data Ecosystem50

Additional file 2 of Transformation and gene editing in the bioenergy grass Miscanthus

植物基因编辑

生物能源作物

Additional file 2. Sequence and location of three gRNAs in the lemon white1 gene. Description: Multiple sequence alignments of lemon white1 orthologs from sorghum and miscanthus and the three gRNAs us

NIAID Data Ecosystem10

Data_Sheet_1_Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes.PDF

植物基因编辑

基因育种

Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added t

NIAID Data Ecosystem60

Estimation for C-to-G editing efficiency induced by CBE and GBE using targeted amplicom sequencing in tobacco

植物基因编辑

碱基编辑效率评估

Estimation for C-to-G editing efficiency induced by CBE and GBE using targeted amplicom sequencing in tobacco

NIAID Data Ecosystem40