Canis lupus capture targets

Island populations are at heightened risk of inbreeding due to reduced mating opportunities with unrelated conspecifics. Extensive inbreeding can result in inbreeding depression (reduced fitness of individuals with related parents). Alexander Archipelago wolves (Canis lupus ligoni) are a geographically isolated subspecies that occur in the Southeast Alaskan panhandle, USA and coastal British Columbia, Canada. Wolves on the Prince of Wales Island complex (POW) in Southeast Alaska are expected to have lower levels of resiliency because they are a small, insular population that has experienced habitat fragmentation and cycles of moderate to heavy harvest. To understand the extent of population structure and inbreeding in Alexander Archipelago wolves, we designed a DNA hybridization capture for wolves and sequenced captured DNA from 58 individuals sampled from across Southeast Alaska during 2002–2016. Estimates of the proportion of the genome in runs of homozygosity (FROH) regardless of run..., , , # Canis lupus capture targets

[https://doi.org/10.5061/dryad.x3ffbg7wh](https://doi.org/10.5061/dryad.x3ffbg7wh)

## Description of the data and file structure

This dataset contains the capture targets used in Zarn et al. (in review), to obtain sequence data from 58 individuals sampled from across Southeast Alaska during 2002–2016, and used to quantify the extent of population structure and inbreeding in Alexander Archipelago wolves. We used a custom hybridization-based capture to target tens of thousands of non-coding SNPs.

We have submitted the study capture targets (\"181102_CanFam31_ZARNwolfSNPsV2_EZ_HX1_capture_targets.bed\"), primary capture targets (\"181102_CanFam31_ZARNwolfSNPsV2_EZ_HX1_primary_targets.bed\"), and the coverage summary (\"181102_CanFam31_ZARNwolfSNPsV2_EZ_HX1_coverage_summary.txt\"). The coverage summary details the reference genome used (CanFam3.1), the number of regions covered, the length of base pairs in the regions, and statistics of probe coverage and estimat...,