K6-Linked Ubiquitylation Signals Reactive Aldehyde-Induced RNA-Protein Crosslink Resolution

Reactive aldehydes induce the formation of RNA-protein crosslinks (RPCs), RPCs in the mRNA stall the ribosome and inhibit translation, RPCs are K6-linked ubiqutylated by RBR-type E3 ligase RNF14, RPCs are resolved in a UBXN1-VCP/p97-dependent manner Overall design: Proteome-wide iCLIP to detect the distribution of RPC across RNAs; RPCs were introduced with 5 uM 4sU + 0.5 J/cm² UVA (365 nm); protein cleanup with SP3 beads; 2 replicates