Natural variation in the atypical resistance gene Pita2 confers broad-spectrum neck blast resistance in rice

Supplementary tables for "Natural variation in the atypical resistance gene Ptr confers broad-spectrum neck blast resistance in rice"

Table S1. Neck blast reactions of the 335 GWAS accessions inoculated with M64-1-3-9-1. Values represent mean values of DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm).*Values represent lesion length (mm) and are classified as resistant (R, 0-7 mm), moderately resistant (MR, >7-30 mm), moderately susceptible (MS, >30-55 mm), or susceptible (S, >55 mm).

Table S2. Neck blast reactions of IRRI elite indica breeding lines inoculated with M64-1-3-9-1. Values represent lesion length (mm) and are classified as resistant (R, 0-7 mm), moderately resistant (MR, >7-30 mm), moderately susceptible (MS, >30-55 mm), or susceptible (S, >55 mm).

Table S3. Modified evaluation scale for neck blast disease in IRRI Plant Pathology Laboratory (Yanoria et al., 2025).

Table S4. Primers used in the qRT-PCR experiments and for potential off-target sequencing in CRISPR-Cas9 mutants.

Table S5. Subpopulation-level neck blast reactions of the 335 GWAS accessions inoculated with M64-1-3-9-1. DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm). *Values indicate number of accessions in the 3K RGP.

Table S6. List of SNPs exceeding the Bonferroni significance threshold value -log₁₀(P) = 6.7.

Table S7. List of SNPs located within the 42.3-kb haploblock on chromosome 12 (Chr12:10,822,390-Chr12:10,864,690). DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm).

Table S8. Neck blast reactions of five Ptr alleles in the GWAS accessions. Values represent mean values of DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm). Genotype information of the A/G SNP at Chr. 12: 10,833,400 is provided for each Ptr alleles.

Table S9. Neck blast reactions of the Ptr and Pi-ta 25 LTH monogenic lines inoculated with 20 differential blast isolates from the Philippines. Values represent lesion length (mm) and are classified as resistant (R, 0-7 mm), moderately resistant (MR, >7-30 mm), moderately susceptible (MS, >30-55 mm), or susceptible (S, >55 mm). LTH was included as susceptible check.

Table S10. Neck and leaf blast reactions of the monogenic line harboring Ptra. The line was inoculated with the 20 differential blast isolates from the Philippines. Values represent lesion length (mm) and are classified as resistant (R, 0-7 mm), moderately resistant (MR, >7-30 mm), moderately susceptible (MS, >30-55 mm), or susceptible (S, >55 mm). LTH was included as susceptible check for neck and leaf blast. ND = No data.