Quasispecies analysis of FU-treated VSV, FMDV and EMCV populations.

aBHK-21 cell monolayers were infected with VSV, FMDV or EMCV at the indicated MOI in the absence or presence of 5-fluorouracil, as detailed in Materials and Methods.
bThe genomic regions sequenced were nucleotides 5902 to 6569 of the L-coding region of VSV, 6800 to 7350 of the 3D (polymerase)-coding region of FMDV, and 6718 to 7403 pf the 3D (polymerase)-coding region of EMCV.
cTiter of VSV, FMDV and EMCV progeny determined at 24 h p.i.
dAverage number of mutations per nucleotide, counted relative to the corresponding consensus nucleotide sequence. The mutation frequency of VSV increased in the presence of FU, and was 1.9-fold higher at low than at high MOI, but the differences were not statistically significant (p = 0.33; χ2 test).
eProportion of different genomic sequences in the mutant spectrum of the quasispecies, calculated as described in Materials and Methods. Variations in Shannon entropy followed, the same trend as variations in mutation frequency but, again, the differences were not statistically significant (see footnote d).
fThe omitted transversion types were not represented in the sequences analyzed.