Data underlying the publication: Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP

This dataset contains data collected for the publication 'Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP' (doi: https://doi.org/10.1101/2023.06.26.546500). The data contains GTPase Glo assay data and SEC-MALS data. GTPase assay data: The purpose of these experiments was to quantify the GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GEF Cdc24 and GAP Rga2, and variants thereof (Cdc24-DH3, GAP domain). We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data where different concentrations of the mentioned proteins, in different combinations, are used. (2) SEC-MALS data: The purpose was to asses the oligomeric state of S.cerevisae proteins Cdc24, Rga2, and the GAP domain of Rga2.

本数据集收录自学术论文《鸟苷酸交换因子（GEF）与GTP酶激活蛋白（GAP）通过寡聚化依赖与协同方式调控Cdc42 GTP酶循环》（doi: https://doi.org/10.1101/2023.06.26.546500）。本数据集包含GTPase-Glo检测数据与尺寸排阻色谱-多角度激光光散射（SEC-MALS）数据。
GTP酶检测实验数据：本系列实验旨在定量分析酿酒酵母（S. cerevisae）GTP酶Cdc42单独存在时，以及与其鸟苷酸交换因子Cdc24、GTP酶激活蛋白Rga2及其变体（Cdc24-DH3、Rga2的GAP结构域）联合存在时的GTP酶活性。本实验采用普洛麦格（Promega）公司的GTPase-Glo检测试剂盒。本次数据包含不同浓度、不同组合的上述蛋白的检测结果。
(2) SEC-MALS数据：本实验旨在评估酿酒酵母蛋白Cdc24、Rga2以及Rga2的GAP结构域的寡聚化状态。