Molluscicidal activity of saponins from tea seeds Camellia oleifera Abel. on golden apple snails Pomacea canaliculata Lamarck

The molluscicidal activity of tea seed extract from Camellia oleifera Abel. against golden apple snails Pomacea canaliculata Lamarck. Showed the LC[supscript 50] values at 6.79 ppm after 24 h. Bioassay-directed fractionation of C. oleifera Abel extract resulted in the isolation of saponins group designated D4 with molecular weight between 1226-1338 Dalton. The bioassay data revealed that at 0.66 ppm D4 had molluscicidal activity with 43.44% mortality against the golden apple snails. In addition, the quantitative analysis of D4 was performed by reversed phase high performance liquid chromatography with UV detector. Optimal chromatographic conditions are C18 column (250x4.60 mm), 40% methanol-0.1% acetic acid in water as mobile phase, flow rate 0.,80 mL/min, wave length detection at 235 mm. Satisfactory validation parameter was obtained: standard calibration was constructed from 10-200 ppm (R [superscript 2]>0.9990). Extract of fruit pericarp from C. oleifera Abel. And extract of commercial tea seed granule was analyzed with HPLC method. The amount of D4 in saponin crude extract from tea seed cake, CU1-MeOH, CU1-H[subscript 2]O, FP-MeOH and FP-H[subscript 2]O were found to be 6.82, 0.43, 0.25 and 1.07 % w/w, respectively.