Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio parahaemolyticus Biofilm Formation

VP biofilm poses a serious threat to food safety and human health. However, there is limited knowledge of transcriptional regulatory mechanism during the biofilm formation of this organism. Hence, the RNA sequencing technique was employed to compare the differences in profiles between floating and biofilm state of VP in this study. Collections of mRNA from floating and biofilm cells were sequenced by studying their biological characteristics. The results showed that there were significant differences in the expression levels of 956 genes in biofilms compared with floating cells. These differences suggested that two-component regulatory system and quorum sensing regulated VP biofilm formation by affecting important factors such as motility, extracellular protein secretion, TRAP transport system and ABC transport system. The present work in RNA-seq serves as a basis for future studies examining the complex network systems that regulate bacterial biofilm formation.