Development of SCNT embryos cultured in media with or without growth factor supplementation.
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Failed-to-be-fertilized oocytes were vitrified and then thawed before evaluation for morphology and SCNT using serum-starved fibroblasts arrested at the G0/G1 stage. After SCNT, reconstituted oocytes were activated using using 5 µM calcium ionophone for 10 min,followed by incubation for 4 h with 2.5 mM 6-dimethylaminopurine, and cultured in media with or without growth factors. Numbers of oocytes/embryos at each experimental stage are shown. *P = 0.187.
创建时间：
2015-12-02
