Data from: Automated workflow for the cell cycle analysis of (non-)adherent cells using a machine learning approach

Understanding the details of the cell cycle at the level of individual
cells is critical for both cellular biology and cancer research. While
existing methods using specific fluorescent markers have advanced our
ability to study the cell cycle in cells that adhere to surfaces, there is
a clear gap when it comes to non-adherent cells. In this study, we combine
a specialized surface to improve cell attachment, the genetically-encoded
FUCCI(CA)2 sensor, an automated image processing and analysis pipeline,
and a custom machine-learning algorithm. This combined approach allowed us
to precisely measure the duration of different cell cycle phases in
non-adherent cells. Our method provided detailed information from hundreds
of cells under different experimental conditions in a fully automated
manner. We validated this approach in two different Acute Myeloid Leukemia
(AML) cell lines, NB4 and Kasumi-1, which have unique cell cycle
characteristics. Additionally, we tested the impact of drugs affecting the
cell cycle in NB4 cells. Importantly, our cell cycle analysis system is
freely available and has also been validated for use with adherent cells.
In summary, this report introduces a comprehensive, automated method for
studying the cell cycle in both adherent and non-adherent cells, offering
a valuable tool for cancer research and drug development.