Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins.

Data from ProteomeXchange, PXD ID: PXD001246. File: GLY_M11_20140408.mzml. Published as part of Mol Cell Proteomics. 2014 Dec 13 . From the Abstract: {{i}} Large scale quantitative evaluation of the tightness of membrane association for non-transmembrane proteins is important for identifying true peripheral membrane proteins with functional significance. Herein, we simultaneously ranked more than 1,000 proteins of the photosynthetic model organism Synechocystis sp. PCC 6803 for their relative tightness of membrane association using a proteomic approach. Using multiple precisely ranked and experimentally verified peripheral subunits of photosynthetic protein complexes as the landmarks, we found that proteins involved in two-component signal transduction systems and transporters are overall tightly associated with the membranes, whereas the associations of ribosomal proteins are much weaker ... {{/i}}