Effect of FL-Fc-DM1 drug on gene expression of THP-1 cells

To eliminate cell-cycle arrested AML cells, we developed a FL-Fc-DM1 drug by conjugating FLT3 liangd Fc fusion protein and mertansine. We used the THP-1 cells as a AML model. The total mRNA of THP-1 cells treated with different concentration of FL-Fc-DM1 were sequenced. Overall design: THP-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS. In the control group, THP-1 cells were cultured for 1 day. In the FL-Fc-DM1 treatment groups, THP-1 cells were treated with FL-Fc-DM1 (1 and 3 nM) for 24 hours. We than analyze the transcriptome differences between different groups.