Solanum nigrum Transcriptome or Gene expression. Solanum nigrum

The seeds of S. nigrum (Sn) were sown under sterile conditions in Petri dishes that contained MS medium and were solidified with 0.8% (w/v) agar. Seven-day-old seedlings were transferred into Hoagland solution and were grown in a sterilized, pathogen-free greenhouse. The cultures were maintained at 22-25°C under a 16-h photoperiod. The treatment with 50 µM CdCl2 was applied to four-week-old seedlings that were grown in Hoagland solution for 24 h. The culture solution was replaced every 3 days. ck, untreated control roots. tr, 50 µM CdCl2 treatment for 24 h.