Genome-wide estrogen-related receptor gamma (ERRg) occupancy in human iPS cell-derived cardiomyocytes (hiPSC-CMs)

Estrogen-related receptor gamma (ERRg) has been shown to control gene expression involved in a broad range of mitochondrial energy metabolism including oxidative phosphorylation, TCA cycle, and fatty acid oxidation. However, ERRg direct targets were not identified in cardiomyocytes. With ERRg ChIP-seq, we found ERRg peaks on the promoter regions of mitochondrial energy metabolic genes as expected. Besides, ERRg extensively distributed the promoter regions of cardiac contractile, ion channels and Ca2+ handling protein genes. Surprisingly, the peaks also were found on non-cardiomyocyte genes and genes expressed in early-stage cardiomyocytes. Overall design: With ChIP-seq, examination of ERRg occupancy on the human genome in wild type hiPSC-CMs and ERRg KO hiPSC-CMs.