Kinetics of the redox reactions in STEAP1 and STEAP2

The data repository includes the kinetic data of the redox reactions in STEAP1 and STEAP2, which are presented in "Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2" by Kehan Chen, Lie Wang, Jiemin Shen, Ah-lim Tsai, Ming Zhou and Gang Wu. The data include: 1) the reduction of ferric STEAP1 by reduced FADH2 and ferrous STEAP2; 2) the reduction of ferric STEAP1 by cytochrome b5 reductase; 3) the reduction of ferric STEAP2 with NADPH; and 4) the oxidation of ferrous STEAP1 and STEAP2 by ferric.NTA.

Methods
All the data was collected under anaerobic conditions. The time-dependent optical changes in the absorbance of the heme in STEAP1 and STEAP2 were monitored using either a diode-array UV-Vis spectrophotometer or a stopped-flow machine with a rapid-scan accessory. In each spectrum, the background absorbance is subtracted. The absorbance at 800 nm is taken as the background in the spectra acquired on the spectrophotometer and the absorbance at 703–708 nm is taken as the background in the data obtained using the stopped-flow rapid-scan method. The time courses of the Soret wavelength of the heme were either extracted from the data of the time-dependent spectral change or acquired on the stopped-flow machine operated in the single wavelength mode. 

For more experimental details, please refer to the Method section in "Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2" by Kehan Chen, Lie Wang, Jiemin Shen, Ah-lim Tsai, Ming Zhou and Gang Wu.