2009 labeled acetate experiment

A methane flux experiment was conducted in 4.5 cm internal diameter cores collected from approximately 5.5 m in Lake GTH 112. Cores were collected and overlying water was replaced with acetate treated water or Control water (Lake GTH 112 hypolimnetic water). Acetate concentrations were 0.2 mM (low acetate) or 2 mM (high acetate) 13C labeled 1-acetate and 13C labeled 2-acetate amendments in GTH 112 hypolimnetic water. Cores were incubated for 4 weeks at ambient Toolik Lake temperature (about 15 deg C),core water was replaced with Lake GTH 112 hypolimnetic water and CH4 flux was measured at 8 deg C over 24 h (ambient O2 condition). Following 24-h flux measurement, overlying water was replaced with N2 purged water (anoxic condition) and CH4 flux was again measured over 24 h. Data report 24 h flux measurement under ambient and anoxic conditions for each treatment (details reported in Hershey et al. 2013 Biogeochemistry 10.1007/s10533-013-9864-y).