Myeloma cells via E-cadherin convert pDCs to become tumor-promoting cells

Analysis of gene expression change induced by myeloma cells in pDCs. The hypothesis tested in the present study was that myeloma cells inhibit pDCs function by direct contact. Results provide important information of gene expression change in the cocultured of pDCs and myeloma, such as IFNs and IFN regulatory genes, TLR9 signaling pathways. PDCs were obtained from human blood cells and cultured alone or with myeloma cell line ARP1 with or without CpG for 12 hours. Due to the sensitivity of pDCs, RNAs were obtained directly from different cell cultures instead of to sort the pDCs from cocultured. Microarray data were analyzed by comparing with different control.