Gcn5 Controls Virulence of the Human Fungal Pathogen Candida albicans through Multiple Regulatory Networks

Transcriptomic analysis of wild type and GCN5-deficient Candida albicans cells prior to and after treatment with Caspofungin 15 min and 45 min was done to determine the transcriptional changes in these strains Overall design: RNA Sequencing was performed of wild type and Gcn5-deficient Candida albicans strains prior to and after treatment with Caspofungin 15 min and 45 min. Cells were grown to the exponential phase in YPD at 30oC. Aliquots were harvested for RNA isolation and to the remaining culturecaspofungin was added to a final concentration of 10 ng/ml. After 15 min and 45 min incubation at 30°C cells were harvested for RNA isolation. Three biological replicates for each time-points of wild-type (wt) treated with caspofungin (CSP, 0, 15 and 45 min), as well as homozygous deletion (gcn5?/?) and two biological replicates for the remaining two time-points of gcn5?/? (CSP, 15 min and 45 min) were sequenced