Analysis of the experimental data in Win and Smolke (2007) [14].

†This arbitrary unit (a.u.) for GFP expression is defined by Win and Smolke [14]. With this definition, the GFP expression level for unengineered mRNA is 50 a.u.. Source of data: SI Figure 13 of Win and Smolke [14]. ‡According to the definition of [R]Rel (18) and the assumption that gene expression level is proportional to steady-state intact mRNA level, and are calculated with the following equations: . ≠The cleavage tendencies (ω) are calculated with equations (25) and (35) for ligand-activated and ligand-inhibited aptazymes, respectively. The value of D used in these calculations is 10 (see text). §The maximum available relative ligand concentrations () are calculated with equations (40) and (41) for ligand-activated aptazymes and ligand-inhibited aptazymes, respectively. *The five constructs that showed high are denoted with *.