In-depth analysis of proteomic and genomic fluctuations during the time course of human embryonic stem cells directed differentiation into beta cells

We describe fluctuations in the correlation between gene expression, its protein abundance, and phosphorylation, which follow beta-cell differentiation protocol perturbations of stem cell fates Overall design: RNA extracts were collected in four replicates of every stage of the differentiation protocol including undifferentiated embryonic stem cells. Deep sequencing was done with Illumina deep sequencing protocol for transcript abundance, variant detection of mammalian samples with 25M reads of polyA modified transcripts