Raw sequencing data and analysis files for the midgut transcriptome and microbiome of Bombyx mori exposed to manganese (Mn)

This dataset contains the raw sequencing data and key analysis files supporting the manuscript titled "Integrated analysis of midgut transcriptome and microbiome reveals manganese-induced dysbiosis driver metabolic disruption and developmental toxicity in <i>Bombyx mori</i>." The research investigates the toxicological effects of dietary manganese (Mn) on the economically important insect, <i>Bombyx mori</i> (Liangguang No. 2 strain). Fifth-instar larvae were fed an artificial diet supplemented with different concentrations of manganese sulfate (MnSO₄) for 48 hours, divided into three groups: a control group (MC, 0 g/kg Mn), a medium-dose group (MM, 15 g/kg Mn), and a high-dose group (MH, 30 g/kg Mn). The dataset is comprised of three main components. First, it includes the raw paired-end FASTQ (.fq) files from 16S rRNA gene (V4 region) amplicon sequencing of the midgut microbiome, containing 21 samples with 7 biological replicates per group (named MCS1-7, MMS1-7, and MHS1-7). Second, it contains the Wiggle format (.wig) files from the midgut tissue RNA-sequencing (RNA-seq) analysis, which represent the genome-wide read coverage depth for visualization purposes, consisting of 9 samples with 3 biological replicates per group (named MCT1-3, MMT1-3, and MHT1-3). Third, the dataset includes the reference genome annotation file (SilkDB 3.0) that was used for the alignment and analysis of the RNA-seq data. This collection of data serves as a valuable resource for studying the interactive responses of the host gut transcriptome and gut microbial community under heavy metal stress, contributing to a deeper understanding of the ecotoxicological mechanisms of manganese in <i>Bombyx mori</i>.