Genome assembly of Triticum turgidum subsp. durum cv. Langdon

Summary of the datasets

Durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husnot) cultivar Langdon is an experimental standard strain that has been used as a parental strain to produce chromosome substitution lines and synthetic hexaploid wheat. We maintain 'Langdon' pure line (strain No.: LPGKU2272) under National BioResource Project Wheat (NBRP-Wheat) by self-pollination.

We constructed a genome assembly of Langdon from about 252 Gbp of HiFi reads using Hifiasm v0.19.8-r603 with additional options '-l 0 -f 39'. The assembly consists of 4,391 contigs (total size: 10,497,834,563 bp, N50: 27,495,971 bp).

Assembly: Triticum_durum.Langdon.hifiasm_assembly.v0.1.fa.gz

We further performed reference-guided scaffolding to assign the contigs into 14 chromosomes of tetraploid wheat using RagTag v2.1.0 software with aligner option 'unimap'. In this scaffolding process, the public sequence of durum wheat cv. Svevo (Svevo.v1; Maccaferri et al., Nat. Genet., 2019) is used as reference genome.

Scaffolded sequence: Triticum_durum.Langdon.ragtag_scaffold.v0.1.fa.gz

Acknowledgement

This work has been conducted under National BioResource Project (NBRP), Ministry of Education, Culture, Sports, Science and Technology, Japan.