Zea mays single cell bisulfite sequencing samples. Zea mays subsp. mays

To access the DNA methylation in single cell level is still a challenge with high resolution and coverage. We developed an efficient method owning high rates for read mapping and genome coverage by using Bisulfite-converted random integrated fragments sequencing (BRIF-seq). Single microspores of maize were isolated and used to test BRIF-seq method.