LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues [hEMB]

Using single-cell genomics, we demonstrated that endogenous LINE-1s are mobilized in human pre-implantation embryos We isolated single cells from the Inner Cell Mass of human pre-implantation embryos that reach the blastocyst stage, amplified their genomes using Multiple Displacement Amplification (MDA), and analyzed the presence of new LINE-1 insertions using the methodology Retrotransposon Capture Sequencing (RC-Seq)