Quantitative image analysis of PMA-treated primary in vitro horizontal basal cells

This dataset contains the CellProfiler-mediated quantitative image analysis described in Barrios-Camacho, CM et al (2024). The purpose of this analysis was to quantify TP63 levels across 12-hours of sustained treatment with 50nM of phorbol 12-myristate 13-acetate. We sampled TP63, CK5, and RELA levels via immunostaining. HBCs were sampled over six different time points. We established nuclear and cytoplasmic boundaries by setting masks with DAPI and CK5. DAPI+ regions were annotated as nuclei, while CK5-DAPI+ regions were annotated as the cytoplasm.