Kinetic properties of homogeneous recombinant wild-type and mutant RubisCOs from A. fulgidus RbcL2 and wild-type RubisCO from T. kodakaraensis RbcL assayed at 83°C.

a Average of at least three independent assays.b Determined at 20 x KRuBP except for the S363V and M295D/S363I and M295D/S363V mutant enzymes which were determined at 4–6 x KRuBP.c Values for WT and M295D previously reported (17) and run in parallel with the other mutant A. fulgidus and T. kodakaraensis enzymes for comparisonKinetic constants were determined as previously described [17].