Deciphering the determinants of recombinant protein yield across the human secretome

Mammalian cells are critical hosts for the production of most therapeutic proteins and many proteins for biomedical research. While cell line engineering and bioprocess optimization have yielded high protein titers of some recombinant proteins, many proteins remain difficult to express. Here we use systems biology methods to deciper the factors influencing yields in Chinese hamster ovary (CHO) cells as they produce proteins from the human secretome. Overall design: Comparative gene expression profiling analysis of RNA-seq data for a representative set of 95 samples each expressing a different human protein in CHO.