ITSoneDB
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ITSoneDB is a comprehensive collection of eukaryotic ribosomal RNA Internal Transcribed Spacer 1 (ITS1) sequences. It is aimed at supporting metabarcoding surveys of fungal and other microbial eukaryotic environmental communities. The sequences were extracted from the European Nucleotide Archive (ENA) and arranged on the NCBI taxonomy tree. ITS1 start and end boundaries were defined by ENA annotations and/or designed by mapping Hidden Markov Model (HMM) profiles of flanking 18S and 5.8S ribosomal RNA coding genes on each sequence
ITSoneDB是一套综合性的真核生物核糖体RNA内部转录间隔区1(Internal Transcribed Spacer 1,ITS1)序列数据库,旨在支撑真菌及其他微生物真核生物环境群落的元条形码(metabarcoding)调查研究。该数据库的序列均从欧洲核苷酸档案库(European Nucleotide Archive,ENA)中提取,并基于NCBI分类学树进行分类排布。ITS1的起始与终止边界既可通过ENA的注释进行定义,也可通过将侧翼18S与5.8S核糖体RNA编码基因的隐马尔可夫模型(Hidden Markov Model,HMM)特征图谱映射至各条序列来确定。




