Genome-wide analysis of mRNA half-lives in Arabidopsis T87 cultured cell.

Gene expression in eukaryotes is modulated at multiple levels from initiation of transcription through mRNA degradation. mRNA degradation is one of the most critical steps on the gene expression (Keene J.D. 2010). In the previous study, transcriptional inhibitor (Actinomycin D) has been used to analyze mRNA degradation. However, transcriptional inhibitor has a disruptive impact on cellular physiology and disturb the precise determination of the RNA degradation rate (half-lives). Recently, genome-wide RNA decay analysis under inhibitor-free condition has been developed. Especially, 5-bromouridine (BrU) is less harmful than other method. Thus, we performed bromouridine immunoprecipitation chase (BRIC) method to determine the mRNA half-lives in Arabidopsis T87 cultured cell.