Genome-wide analysis of the role of S276 phosphorylation of p65-NFkappaB on the regulation of gene expression in response to EGF or IL-1 treatment.. Homo sapiens

Genome-wide gene expression levels of were measured by microarray in wildtype (WT) or S276A-p65 HMECs treated with or without 100 ng/mL EGF or 10 ng/mL IL-1 for 4 h. Overall design: To interrogate the role of S276 phosphorylation in NF-kappaB genomic regulation in response to EGF or IL-1 stimulation, using the gene editing approach CRISPR, we introduced the genetic mutation S276A in the RELA gene in HMECs by means of HDR repair of the Cas9-induced DSB.