Next generation sequencing for quantitative transcriptomic analysis of subpopulations of BM cells from young Hopx-wildtype and Hopx-knockout mice.

Hopx conditional knockout mouse (Hopx-/-) was generated. We then studied the biological effects of Hopx knockout on the hematopoietic system of young mice, via whole-transcriptome RNA-seq. Gene expression profiling of subpopulations of bone marrow cells, including long-term HSC (LT-HSCs), type 2 multipotent progenitors (MPP2), type 3 multipotent progenitors (MPP3), of young Hopx-wildtype and Hopx-knockout mice, was generated by RNA sequencing.