Mouse_Tbx5_del_CreERT2_bulk_RNAseq

Haploinsufficiency of transcriptional regulators causes human congenital heart disease (CHD), suggesting that reduced transcription factor dosage causes a gene regulation imbalance. Here, we define transcriptional responses to reduced TBX5 in embryonic mouse hearts. Embryonic heart mRNA profiles at E10.5 of wildtype (WT), Tbx5 CreERT2/+, Tbx5 del/+ and Tbx5 del/CreERT2 were generated by deep sequencing, in quadruplicate.转录调节因子（transcriptional regulators）的单倍剂量不足可引发人类先天性心脏病（congenital heart disease, CHD），这提示转录因子剂量降低会导致基因调控失衡。本研究阐明了小鼠胚胎心脏中TBX5剂量降低所诱导的转录应答反应。我们通过深度测序获取了野生型（wildtype, WT）、Tbx5 CreERT2/+、Tbx5 del/+ 及 Tbx5 del/CreERT2 小鼠胚胎心脏在胚胎发育第10.5天（E10.5）的mRNA表达谱，所有样本均设置四重生物学重复。