Hydrogel-Based Implantable System for Local Delivery of Temozolomide in Postsurgical Brain Cancer Therapy - research data

Data generated during studies on the hydrogel-based implantable system for local delivery of temozolomide are provided. The raw experimental dataset consists of the physicochemical evaluation of developed hydrogel-based systems (the degradation, gel fraction and swelling experiments). In addition, data from model drug release experiments performed under physiological conditions are presented. Moreover the selected dataset regarding the biological evaluation in vitro was also provided.

Human polymorphonuclear neutrophils were analyzed for the formation of neutrophil extracellular traps (NETs) using fluorescence microscopy. Microscopy images were captured using a fluorescence microscope (Olympus IX83) at a magnification of 40x. The following fluorescent markers were used: DAPI (cell nuclei) and Alexa Fluor 546 (myeloperoxidase). Exposure settings and laser intensities were consistent across all samples. The images were processed for noise reduction and contrast enhancement. The raw images were processed using ImageJ (1.54f) software. The fluorescence range was standardized across all images, and signal intensity was normalized. The files were saved in TIFF format to preserve high resolution. TIFF file names follow the convention: type of material_temozolomide grafting_ time point of extract collection (e.g. 541_Ctr_4h)

Excel files were exported from the LEGENDplex™ software (BioLegend, USA). Calculations in the LEGENDplex™ software were performed according to the default settings (the gating strategy was based on the LEGENDplex™ software algorithm, without any changes made by the data analysis personnel). Excel file names follow the convention: species_cell type_report_date of report export (e.g. Human_hPMN_hPBMC_report_2024-11-26)