Expression data from HER2 L755S, PIK3CA E545K, and co-cultured cells in the MCF-10A background

When co-cultured, HER2 L755S mutant cells are induced to proliferate when coming into close contact with PIK3CA E545K mutant cells We used a microarray to compare transciptome-wide expression levels between HER2 L755S cells, PIK3CA E545K cells, and co-cultures containing both cell types. HER2 L755S and PIK3CA E545K mutant cells in the MCF-10A background were grown separately, and in co-cultures. When co-cultures reached ~75% HER2 L755S cells, all samples were harvested. Each single control and the co-cultures were grown in quadruplicate. The three experimental groups were HER2 L755S single controls, PIK3CA E545K single controls, and co-cultures containing ~75% HER2 L755S and 25% PIK3CA E545K.