Transcriptional regulation of ES cell by FGF signalling. Transcriptional regulation of ES cell by FGF signalling

FGF4 paracrine signaling through FGF receptors is believed to induce Erk activity and promotes the exit from pluripotency. However, we have recently observed that FGF4 and FGF2 have opposing effects on mES cell fate. The aim of this study was to understand the underlying transcriptional changes associated with these opposing actions. Overall design: Mouse embryonic stem cells were grown in a naïve state of pluripotency using 2iLIF media and subsequently stimulated with FGF2 in N2B27 media for 24hrs and 48hrs.