<b>Comprehensive analysis of B3 family genes in pearl millet (</b><b><i>Pennisetum glaucum</i></b><b>) and the negative regulator role of </b><b><i>PgRAV-04</i></b><b> in drought tolerance</b>Untitled Item

Supplementary Table 1 The list of Primer used in this studySupplementary Table 2 The list of Identification and analysis of PgB3sSupplementary Table 3 The list of B3 homologous genes that have been annotatedSupplementary Table 4 The list of Arabidopsis thaliana as a homologous species for interactions analysi of PgB3s proteinsSupplementary Table 5 The list of mean FPKM values of PgB3s at different time points under drought treatmentSupplementary Table 6 The list of mean FPKM values of PgB3s at different time points under high-temperature treatmentSupplementary Table 7 The physiological experiment data of three transgenic lines and the WT plants<b>Figure S1</b><b> </b>Phylogenetic relationship of the B3 superfamilies of At (<i>Arabidopsis thaliana</i>), Os (<i>Oryza sativa</i>), and Pg (<i>Pennisetum glaucum</i>). The four colors represent four different subfamilies of the B3 family.<b>Figure S2</b><b> </b>Transformation process of transgenic tobacco. A: preincubate; B: induction; C: screening; D: differentiation; E: rooting.<b>Figure S3</b><b> </b>Identification of transgenic tobacco by different techniques. A: PCR identification of DNA extracted from transgenic plants, M: DL2000 DNA Marker; 1~18 is the PgRAV-04 OE plants; + is a positive control; − is a negative control; WT is a wild-type. B: RT-PCR identification of genetically modified tobacco，OE1-OE6 is PgRAV-04 OE plants, WT is a wild-type. C: Real-time fluorescence quantitative PCR identification of transgenic tobacco, <i>Actin</i> was amplified as an internal reference gene. Data are mean ± SD of three replicates