Fate Mapping of cGAS-activated Cells by Tracing Genome and Protein-Protein Interaction Networks of cGAS

We created single clones of BEAS2B cells stably expressing low-levels of DamID-alone and DamID-cGAS constructs using lentiviral particles. Two stable clones expressing low and comparable levels of DamID-alone and DamID-cGAS were used for subsequent experiments. We either mock- or treated with DNA damaging agents, purified genomic DNA from one-three million cells and then specifically amplified m6A-methylated DNA. Subsequently, we processed the PCR amplified DNA for HiFi genome sequencing and obtained raw sequencing data files in the form of fastq files. All sequencing was performed as paired-end 150 bp reads generated by the UT Southwestern Medical Center, Genomic sequencing Core using an Illumina HiSeq 2500 v3 sequencing system.