The asymmetric gene expression analysis in the hypocotyls of soybean (Glycine max) after gravistimulation

To identify genes that are differentially expressed on the two sides of hypocotyls during the gravitropic response, RNA-seq was performed using the upper and lower sides of dark-grown soybean hypocotyls after gravistimulation. Three-day-old etiolated soybean seedlings were treated with gravitropic stimulation for 0, 1, or 12 h. Gravistimulation was carried out by rotating the plates with vertically grown seedlings. Harvested hypocotyls were separated into two parts, the lower side and the upper side, along the midline. At the indicated timepoints, the two sides of the hypocotyls were collected separately for RNA extraction and sequencing. Two biological replicates for each time point were prepared for RNA-seq analyses. HYDG means the cotyledons facing down and HYUG means the cotyledons facing up.