Invasion ability of glioblastoma cells invading from spheroid cultures.

(A, B) The GB cells form late transfers of spheroids which invaded thorough the Matrigel-coated filter to the lower surface of the filter of invasion chamber stained for SA-β-Gal activity (A) and with DAPI (B). (C) Box-whisker plots (mean, std. error and min/max values) showing percentage of invading SA-β-Gal (+) and SA-β-Gal (−) cells from early and late transfers of spheroids. The results of the statistical analysis (using Mann-Whitney U test) are presented for each case separately and for the cumulative group in the table below.