Influenza type A and type B detector tile sequences and RPM-Flu assay-based identification of gene sequences by RPM-Flu assay of FluMist trivalent vaccine.

aNumber of observed single base polymorphisms (SNPs), as single base mismatches of detector and specimen gene sequences that are flanked by consensus base calls/number of specific base calls. The A/H1N1 detector tile and vaccine specimen hemagglutinin and neuraminidase gene sequences are concordant (bold font, underlined).
bThe original RPM-Flu analysis on 21 Aug 2007 returned four best matching neuraminidase gene sequence records, as B/Yamagata/1246/2003, B/Taiwan/102/2005, B/Bangkok/460/2003, and B/Roma/4/2002. When subjected to BLAST analysis again on 09 Nov 2008, two more best matching neuraminidase gene sequence records were returned, as B/Taiwan/34/2004 and B/Jilin/20/2003. The actual influenza type B vaccine strain was B/Jilin/20/2003, but its neuraminidase gene sequence was not entered into the GenBank database until 21 Jul 2008.