Gene expression profiling of THP-1 cells on treatment with vitamin C

The present study reports the temporal transcriptional response of THP-1 cells to physiological levels (100 μM) of vit C treatment. An extensive modulation of gene expression occurs from early to late time-points, which is reflected in a gamut of functional responses regulated by vit C, as determined by enrichment analysis. The study reveals the pleiotropic activity of vit C with diverse implications on biological processes. vit C mediates responses that encompass a range of cellular processes, and span regulation of gene expression, signal transduction, cell cycle, immune system, cAMP metabolic process and signaling and ion homeostasis. Thus vit C modulates a plethora of physiological functions, that emphasize its key role in maintaining equilibrium. Agilent one-color experiment,Organism: Homo sapiens ,Agilent Custom Human Whole Genome Array 8x60k designed by Genotypic Technology Private Limited (AMADID: 027114) Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)