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Regulation of cytoplasmic calcium is crucial both for proper neuronal
function and cell survival. The concentration of Ca2+ in cytoplasm of a
neuron at rest is >10,000 times lower than in the extracellular
space, pointing to the importance of the transporters that extrude
intracellular Ca2+. The family of plasma membrane calcium-dependent
ATPases (PMCAs) represent a major component of the Ca2+ regulatory
system. However, little information is available on the regional and
cellular distribution of these calcium pumps. We used
immunohistochemistry to investigate the distribution of each of the four
PMCA isoforms in the rat brain.

细胞质钙离子的调控对于神经元的正常功能和细胞的存活至关重要。在静息状态下，神经元细胞质中的钙离子浓度相较于细胞外空间高出超过10,000倍，这一现象凸显了排出细胞内钙离子的转运蛋白的重要性。血浆膜钙离子依赖性ATP酶（PMCAs）家族构成了钙离子调节系统的核心组成部分。然而，关于这些钙泵在区域和细胞层面的分布信息却十分有限。本研究采用免疫组织化学方法，对大鼠脑部四种PMCA异构体的分布进行了详细探究。