Cnidarian cell type diversity revealed by whole-organism single-cell RNA-seq analysis

We performed single-cell transcriptome analysis (using MARS-seq) in the cnidarian Nematostella vectensis. Additionally we performed bulk ATAC-seq experiments on wild-type specimens, as well as ELAV::mOrange reporter transgenic lines (separating ELAV+ and ELAV- cell populations, representing neurons and non-neurons, respectively). Multiple animals (20-25) were dissociated and pooled before sorting single-cells for scRNAseq. 200,000 cells were used in bulk ATAC-seq experiments.