Distribution of the different categories of artefacts found in the panFMDV-5UTR PCR reactions using normal or hot-start dNTPs.
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https://figshare.com/articles/dataset/Distribution_of_the_different_categories_of_artefacts_found_in_the_panFMDV-5UTR_PCR_reactions_using_normal_or_hot-start_dNTPs_/4006062
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资源简介:
RNA samples from different FMDV isolates (n = 12) were amplified with non-tailed or tailed primers using either normal or hot-start dNTPs. RT-qPCR products were analysed by high-throughput sequencing to study the contribution of each primer to the formation of PCR artefacts. Artefacts were classified into 4 categories based on the primer sequences present on both ends (FWD: forward, REV: reverse). The table lists the mean proportion and 95% confidence interval of each artefact category.
创建时间:
2016-10-12



