Irani2015 - Genome-scale metabolic model of P.pastoris N-glycosylation

Irani2015 - Genome-scale metabolic model of
P.pastoris N-glycosylation

This model is described in the article:

Genome-scale metabolic model
of Pichia pastoris with native and humanized glycosylation of
recombinant proteins.

Irani ZA, Kerkhoven E, Shojaosadati
SA, Nielsen J.
Biotechnol. Bioeng. 2015 Oct;
Abstract:

Pichia pastoris is used for commercial production of human
therapeutic proteins, and genome-scale models of P. pastoris
metabolism have been generated in the past to study the
metabolism and associated protein production by this yeast. A
major challenge with clinical usage of recombinant proteins
produced by P. pastoris is the difference in N-glycosylation of
proteins produced by humans and this yeast. However, through
metabolic engineering a P. pastoris strain capable of producing
humanized N-glycosylated proteins was constructed. The current
genome-scale models of P. pastoris do not address native nor
humanized N-glycosylation, and we therefore developed
ihGlycopastoris, an extension to the iLC915 model with both
native and humanized N-glycosylation for recombinant protein
production, but also an estimation of N-glycosylation of P.
pastoris native proteins. This new model gives a better
predictions of protein yield, demonstrates the effect of the
different types of N-glycosylation of protein yield, and can be
used to predict potential targets for strain improvement. The
model represents a step towards a more complete description of
protein production in P. pastoris, which is required for using
these models to understand and optimize protein production
processes. This article is protected by copyright. All rights
reserved.

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