Transcriptome analysis of developing mammalian postnatal intestinal crypts and villi

Villar and intervillar epithelial cells were FACs isolated from P0, P3, P6, or P10 CD1 mouse medial small intestine. Three biological replicates were used for e ach population, and expression profiles were determined using Illumina HiSeq. Comparison of the sample groups allowed for identification of compartment-specific and stage-specific genes. Overall design: 8 distinct samples, with three replicates each. Crypt (Epcam+/CD44+) and Villar (Epcam+/CD44-) populations for CD1 mice at P0, P3, P6, P10.