Bacteroides thetaiotaomicron ameliorates colon inflammation in preclinical models of Crohn’s Disease [Bacteroides]. Bacteroides thetaiotaomicron ameliorates colon inflammation in preclinical models of Crohn’s Disease [Bacteroides]

Analysis of the Bacteroides thetaiotaomicron(BT) transcriptome during co-culture with Caco-2 intestinal epithelial cells To identify potential bacterial protein(s) involved in the anti-inflammatory effect of BT in colitis, BT was incubated with Caco-2 human intestinal epithelial cells for 2 hours, and bacterial gene expression was assessed on a Bacteroides thetaiotaomicron VPI-5482 specific microarray. Forty-three BT genes were up-regulated by five-fold or more and of these, twenty genes encoded hypothetical proteins. Overall design: Caco-2 cells cultured in 35mm dishes were treated with flagellin of Salmonella enteritidis, phorbol-12-myristate-13-acetate or interleukin 1. BT was added at 109 CFU/mL and incubated for 2h. Bacterial culture was then removed and RNA was processed for hybridization to a Bacteroides thetaiotaomicron VPI-5482 specific microarray.