Cpxm2 as a novel candidate for cardiac hypertrophy and failure in hypertension [Illumina]

To identify novel targets in left ventricular (LV) hypertrophy (LVH) we initiated comparative transcriptome analysis between genetic models derived from stroke-prone spontaneously hypertensive rats (SHRSP) and Fischer (F344). Previous genetic studies in the SHRSP/F344 model confirmed the presence of a major quantitative trait locus (QTL) for LV mass on rat chromosome (RNO) 1. We assigned carboxypeptidase X 2 (Cpxm2) to a genetic locus for LV mass identified in SHRSP. We assigned carboxypeptidase X 2 (Cpxm2) to a genetic locus for left ventricular mass. Male SHRSP and F344 rats were obtained. To develop the consomic strain SHRSP-1F344, we crossed the SHRSP strain with the F344 strain in accordance with our linkage results and introgressed the whole rat chromosome (RNO)1 from F344 into the SHRSP background as previously described. In that model, we performed whole transcriptome analysis on LV tissue of 14 weeks old male F344, SHRSP-1F344 and SHRSP (n = 8, respectively) using Illumina RatRef-12 Expression BeadChips.